3MO2
human G9a-like (GLP, also known as EHMT1) in complex with inhibitor E67
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.700, 163.000, 69.300 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 31.830 - 2.490 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fpd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.830 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.095 | 0.384 |
| Number of reflections | 43245 | |
| <I/σ(I)> | 12.8 | 2.6 |
| Completeness [%] | 94.4 | 94.3 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 14% polyethylene glycol 4000, 9% isopropanol, and 12% dimethyl sulfoxide, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
