3MNP
Crystal structure of the agonist form of mouse glucocorticoid receptor stabilized by (A611V, V708A, E711G) mutations at 1.50A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Collection date | 2009-07-13 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 72.089, 72.089, 128.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.380 - 1.500 |
| R-factor | 0.1844 |
| Rwork | 0.183 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mno |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.131 |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 55503 | |
| <I/σ(I)> | 19.9 | 2.26 |
| Completeness [%] | 91.7 | 66.4 |
| Redundancy | 4.05 | 1.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 0.1 M Tris pH 8.5, 0.1 M sodium thiocyanate, 9 % PEG 10000, VAPOR DIFFUSION, SITTING DROP |
