3MIP
I-MsoI re-designed for altered DNA cleavage specificity (-8GCG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-08-01 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.539, 42.611, 71.524 |
| Unit cell angles | 96.45, 107.04, 108.56 |
Refinement procedure
| Resolution | 22.959 - 2.400 |
| R-factor | 0.2316 |
| Rwork | 0.226 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.178 |
| Number of reflections | 17356 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 96.7 | 95.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 20% PEG 4000, 100mM Tris, 20mM NaCl, 5mM CaCl2, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
