3MI0
Crystal Structure of Mycobacterium Tuberculosis Proteasome at 2.2 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 170.374, 118.436, 195.249 |
| Unit cell angles | 90.00, 113.02, 90.00 |
Refinement procedure
| Resolution | 24.950 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fhg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.117 | 0.641 |
| Number of reflections | 362039 | |
| <I/σ(I)> | 9.7 | 1.4 |
| Completeness [%] | 94.3 | 82.7 |
| Redundancy | 4.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 277 | 13.2% PEG 6000, 60mM sodium citrate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
