3MGS
Binding of Cesium ions to the Nucleosome Core particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.93 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.510, 109.668, 182.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.070 - 3.150 |
| R-factor | 0.21635 |
| Rwork | 0.216 |
| R-free | 0.23756 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kx5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (Rigid Body) |
| Refinement software | REFMAC (Rigid Body) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.070 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.091 | 0.477 |
| Number of reflections | 36839 | |
| <I/σ(I)> | 17.8 | 3.1 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 85mM MnCl2, 60mM KCl, 40mM K-cacodylate , pH 6.0, VAPOR DIFFUSION, temperature 291K |
