3MGH

Binary complex of a DNA polymerase lambda loop mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU
Wavelength(s)	1.504
Spacegroup name	P 21 21 2
Unit cell lengths	95.998, 190.886, 58.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.459 - 2.400
R-factor	0.212
Rwork	0.209
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.183
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	47103
Completeness [%]	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	277	2-propanol, pH 6.5, hanging drop, temperature 277K