3MC6

Crystal structure of ScDPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2007-11-27
Detector	PSI PILATUS 6M
Spacegroup name	F 2 2 2
Unit cell lengths	151.100, 156.860, 201.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.920 - 3.150
R-factor	0.243
Rwork	0.241
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	REFINED MODEL OF STSPL MUTANT K311A (DIMERIC)
RMSD bond length	0.002
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	3.320
High resolution limit [Å]	3.150	3.150
Rmerge	0.087	0.710
Number of reflections	20848
<I/σ(I)>	15.1	2.6
Completeness [%]	99.9	100
Redundancy	6.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7	293	PEG 4000 18%, 0.1 M Na-cacodylate (HOAc) pH 6.7, 0.2 M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K