3MBI
Crystal structure of the phosphoribosylpyrophosphate (PRPP) synthetase from Thermoplasma volcanium in complex with ADP-Mg2+ and ribose 5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.599, 61.841, 127.938 |
| Unit cell angles | 90.00, 103.94, 90.00 |
Refinement procedure
| Resolution | 25.470 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lpn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.002 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_343)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.471 | 1.860 |
| High resolution limit [Å] | 1.797 | 1.800 |
| Rmerge | 0.120 | 0.710 |
| Number of reflections | 105923 | |
| <I/σ(I)> | 11.8 | 1.8 |
| Completeness [%] | 97.8 | 96.4 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 30% PEG600, 0.1M HEPES, 10% MPD, 0.1% LDAO, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
