3M85

Archaeoglobus fulgidus exosome y70a with RNA bound to the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.006
Spacegroup name	P 43 2 2
Unit cell lengths	138.020, 138.020, 262.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.984 - 3.000
R-factor	0.1995
Rwork	0.191
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ab1
RMSD bond length	0.009
RMSD bond angle	1.256
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_129))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	51203
Completeness [%]	98.9	98.9
Redundancy		7.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1M Na Acetate, pH 4.6, 30% MPD, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K