3M84

Crystal Structure of Phosphoribosylaminoimidazole Synthetase from Francisella tularensis

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-21
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	62.708, 90.560, 118.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.751 - 1.699
R-factor	0.155
Rwork	0.154
R-free	0.18300
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.208
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.699	1.699
Number of reflections	72851
<I/σ(I)>	18.3	4.3
Completeness [%]	96.7	75.3
Redundancy	3.6	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.2M Litium Sulfate; 0.1M Tris pH 7.0; 2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K