3M5A
SET7/9 Y245A in complex with TAF10-K189me3 peptide and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.108, 83.108, 95.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.140 - 1.750 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f69 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.080 | 0.049 | 0.425 |
| Number of reflections | 38976 | ||
| <I/σ(I)> | 18.1 | ||
| Completeness [%] | 99.9 | 99.3 | 99.8 |
| Redundancy | 6.3 | 6.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 1.7 M Ammonium Sulfate, 0.1 M Bis-Tris pH 6.6, 0.0025 M CoCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
