3M58
SET7/9 Y245A in complex with TAF10-K189me1 peptide and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.210, 83.210, 95.264 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.710 - 1.400 |
| R-factor | 0.17613 |
| Rwork | 0.175 |
| R-free | 0.19784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f69 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.393 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
| Rmerge | 0.048 | 0.033 | 0.432 |
| Number of reflections | 74675 | ||
| <I/σ(I)> | 22.1 | ||
| Completeness [%] | 98.7 | 90.6 | 96.7 |
| Redundancy | 7.4 | 6.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.9 M Ammonium Sulfate, 0.1 M Bis-Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
