3M53
SET7/9 in complex with TAF10 peptide and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.746, 83.746, 96.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.310 - 1.850 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f69 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.474 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
| Rmerge | 0.062 | 0.039 | 0.506 |
| Number of reflections | 33714 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 10.4 | 9.9 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 1.8 M Sodium Citrate, 0.1 M Imidazole pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
