3M2M
Rat galectin-1 complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1993-11-02 |
Detector | SIEMENS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 40.100, 58.600, 121.600 |
Unit cell angles | 101.10, 91.60, 110.90 |
Refinement procedure
Resolution | 36.000 - 2.950 |
R-factor | 0.233 |
Rwork | 0.206 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.330 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MERLOT |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 3.080 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 15682 | |
Completeness [%] | 73.7 | 42.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 30mM NaOAc, PEG 4K 15-17%, 4mM 2-mercaptoethanol, 100mM lactose, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |