3LZF
Crystal Structure of Fab 2D1 in Complex with the 1918 Influenza Virus Hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2009-03-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0333 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 161.745, 161.745, 143.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.690 - 2.800 |
| R-factor | 0.23154 |
| Rwork | 0.230 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.784 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 53366 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 98.4 | |
| Redundancy | 10.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 16% PEG1000, 100mM PIPES pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
