3LZD
Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.695, 80.525, 162.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3lzc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.062 | 0.241 |
| Number of reflections | 41054 | |
| <I/σ(I)> | 8.2 | 2.41 |
| Completeness [%] | 94.3 | 66.4 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 299 | 18% PEG 400, 0.06 M 2-(N-morpholino)ethanesulfonic acid, 0.12 M lithium sulfate, 3% ethylene glycol, pH 6.5, vapor diffusion, hanging drop, temperature 299K |
