3LYR
Human Early B-cell Factor 1 (EBF1) DNA-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 134.218, 134.218, 72.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.120 - 2.510 |
| R-factor | 0.23332 |
| Rwork | 0.233 |
| R-free | 0.27435 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.120 | 2.640 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.064 | 0.317 |
| Number of reflections | 13523 | |
| <I/σ(I)> | 39.3 | 12.8 |
| Redundancy | 27.8 | 28.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 277 | 0.1M MES, 2.0M Ammonium Sulphate, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
