3LYF

Crystal Structure of the Rift Valley Fever Virus Nucleocapsid Protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794,0.9796
Spacegroup name	P 1
Unit cell lengths	67.110, 69.630, 80.620
Unit cell angles	78.45, 69.72, 60.97

Refinement procedure

Resolution	46.510 - 1.930
R-factor	0.21361
Rwork	0.211
R-free	0.25428
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.293
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.930	1.930
Rmerge	0.080	0.430
Number of reflections	87736
<I/σ(I)>	9.6	3.1
Completeness [%]	97.1	95.7
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277.15	26% PEG 3350, 100 mM Na/K phosphate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
1	VAPOR DIFFUSION, HANGING DROP	5.5	277.15	26% PEG 3350, 100 mM Na/K phosphate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K