3LWW

Structure of an open and closed conformation of Human Importin Beta bound to the Snurportin1 IBB-domain trapped in the same crystallographic asymmetric unit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.918
Spacegroup name	P 1 21 1
Unit cell lengths	101.650, 101.735, 101.963
Unit cell angles	90.00, 110.88, 90.00

Refinement procedure

Resolution	14.957 - 3.150
R-factor	0.2663
Rwork	0.264
R-free	0.31290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 2P8Q
RMSD bond length	0.003
RMSD bond angle	0.716
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	3.150	3.150
Number of reflections	32721
<I/σ(I)>	16.3
Completeness [%]	99.2	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	18% PEG 8000, 50 mM sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	18% PEG 8000, 50 mM sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K