3LW8
Shigella IpgB2 in complex with human RhoA, GDP and Mg2+ (complex A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.590, 101.600, 97.030 |
| Unit cell angles | 90.00, 96.43, 90.00 |
Refinement procedure
| Resolution | 48.200 - 1.850 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s1c partially refined structure of free IpgB2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.423 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.052 | 0.492 |
| Number of reflections | 133487 | 9777 |
| <I/σ(I)> | 15.11 | 2.5 |
| Completeness [%] | 98.4 | 97.8 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% (w/v) PEG 3350, pH 7.5, vapor diffusion, hanging drop, temperature 277K |
