3LW8

Shigella IpgB2 in complex with human RhoA, GDP and Mg2+ (complex A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Transforming protein RhoA	polymer	185	20747.7	4	UniProt (P61586) Pfam (PF00071) In PDB	Homo sapiens (human)	H12
2	E, F, G, H	IpgB2	polymer	192	21936.0	4	UniProt (Q9AJW7) Pfam (PF03278) In PDB	Shigella flexneri	IpgB2, probably secreted by the Mxi-Spa secretion machinery
3	A, B, C, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
4	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5		water	water		18.0	1766	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 181 (UniProt: P61586)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Leu -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

E, F, G, H: 1 - 188 (UniProt: Q9AJW7)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Asp 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	170734.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1870.0
All*	Total formula weight	172604.8

*Water molecules are not included.