3LVW
Glutathione-inhibited ScGCL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 118.127, 118.127, 165.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.500 |
| R-factor | 0.20171 |
| Rwork | 0.199 |
| R-free | 0.25054 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ig5 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 0.533 |
| Number of reflections | 40046 | |
| <I/σ(I)> | 9.7 | 3 |
| Completeness [%] | 97.1 | 96.1 |
| Redundancy | 8.77 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 100mM Hepes, pH 6.8, 14-20% PEG 400, 5mM GSH, 5mM MgCl2 , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
