3LVL

Crystal Structure of E.coli IscS-IscU complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	77.647, 77.647, 355.986
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.489 - 3.000
R-factor	0.227
Rwork	0.225
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1P3W and 2Z7E
RMSD bond length	0.004
RMSD bond angle	0.654
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.093	0.053	0.285
Number of reflections	11488
<I/σ(I)>	12.1
Completeness [%]	83.4	96.8	36.5
Redundancy	16.6	18	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	295	16% PEG 8000, 0.2M Sodium Nitrate, 4% Glycerol, 0.1M Bicine pH 9.0, vapor diffusion, sitting drop, temperature 295K