3LRG
Structure of anti-huntingtin VL domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.680, 107.680, 50.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.17181 |
| Rwork | 0.170 |
| R-free | 0.19669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mfa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.373 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.072 | 0.264 |
| Number of reflections | 19201 | |
| <I/σ(I)> | 32.7 | 11.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 15 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.8 | 293 | 1.2 M Ammonium sulfate, 0.2 M Sodium chloride, 0.1 M CHES-NAOH, pH 9.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
