3LQX
SRP ribonucleoprotein core complexed with cobalt hexammine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.818, 77.765, 32.735 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| Rwork | 0.244 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dul |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.930 |
| Number of reflections | 47287 |
| Completeness [%] | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50 mM Na-MES, 200 mM potassium acetate, 5 mM cobalt hexammine, 10% isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
