3LPH
Crystal structure of the HIV-1 Rev dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 115.801, 115.801, 81.163 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.136 - 2.500 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.26130 |
| Structure solution method | MAD; MOLECULAR REPLACEMENT |
| Starting model (for MR) | An initial model was generated using MAD data sets. This lower resolution model was used for molecular replacement with the final native data set. |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.677 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.051 | 0.507 |
| Number of reflections | 11561 | |
| <I/σ(I)> | 35.2 | 3.4 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM Tris pH 8.0, 50 mM NaCl, 1.45-1.55 M (NH4)2SO4, 3% PEG1000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
