3LMV
D-Tyr-tRNA(Tyr) Deacylase from plasmodium falciparum in complex with hepes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.650, 52.950, 89.680 |
| Unit cell angles | 75.40, 74.20, 86.10 |
Refinement procedure
| Resolution | 29.243 - 2.833 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lmt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.920 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.062 | 0.313 |
| Number of reflections | 20391 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 95.8 | 79.5 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M TRIS/HEPES, 25-30% PEG3350, pH 7.5-8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
