3LKZ
Structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.363, 65.740, 77.119 |
| Unit cell angles | 112.01, 102.65, 90.19 |
Refinement procedure
| Resolution | 38.880 - 2.000 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2oy0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | 0.333 |
| Number of reflections | 38704 | |
| <I/σ(I)> | 5.3 | 2.2 |
| Completeness [%] | 81.2 | 37.6 |
| Redundancy | 1.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 13 TO 15% PEG4000, 5% ISOPROPANOL, 0.1M SODIUM CITRATE, PH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
