3LK3
Crystal structure of CapZ bound to the CPI and CSI uncapping motifs from CARMIL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2008-11-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.124, 115.535, 61.675 |
Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.680 |
R-factor | 0.1601 |
Rwork | 0.155 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lk2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.140 |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.270 | 2.800 |
High resolution limit [Å] | 2.680 | 2.700 |
Rmerge | 0.049 | 0.140 |
Number of reflections | 17727 | |
<I/σ(I)> | 29.7 | 8.9 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M KCl, 20% (w/v) PEG 3350 , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |