3LHP

Crystal structure of HIV epitope-scaffold 4E10_D0_1ISEA_004_N 4E10 Fv complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	107
Detector technology	CCD
Collection date	2008-03-10
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	52.977, 88.623, 149.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.990 - 2.700
R-factor	0.24732
Rwork	0.243
R-free	0.33503
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Computationally-derived model of the epitope-scaffold Fv complex with the Fv based on PDB ID 1TZG.
RMSD bond length	0.009
RMSD bond angle	1.132
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.540	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.102	0.405
Number of reflections	19356
<I/σ(I)>	7.1	2.2
Completeness [%]	96.2	85.5
Redundancy	3.45	2.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 2000 MME, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K