3LH0
Crystal structure of the 53BP1 tandem tudor domain in complex with p53K372me2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-11 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.700, 78.280, 36.540 |
| Unit cell angles | 90.00, 122.18, 90.00 |
Refinement procedure
| Resolution | 30.927 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g3r |
| RMSD bond length | 0.033 |
| RMSD bond angle | 1.896 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 2009_02_15_2320_3)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.927 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.188 |
| Number of reflections | 10439 | |
| <I/σ(I)> | 21.9 | 6.6 |
| Completeness [%] | 97.6 | 95.8 |
| Redundancy | 3.66 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M HEPES-Na pH 7.0, 2% PEG 400 and 2.4 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
