3LGD
Crystal structure of human adenosine deaminase growth factor, adenosine deaminase type 2 (ADA2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.212, 73.099, 80.662 |
| Unit cell angles | 113.38, 94.18, 92.16 |
Refinement procedure
| Resolution | 39.680 - 2.000 |
| R-factor | 0.18705 |
| Rwork | 0.185 |
| R-free | 0.23397 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 101215 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 97.4 | 96.9 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.04 | 293 | 40% MPD, 5% PEG 8000, 0.1M cacodylate, pH 6.04, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
