3LCS
Crystal Structure of the Anaplastic Lymphoma Kinase Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-08-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.941, 56.690, 104.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.080 - 1.950 |
| R-factor | 0.19584 |
| Rwork | 0.194 |
| R-free | 0.22946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l9p |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.727 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 0.831 |
| Number of reflections | 29513 | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 94.9 | 95.1 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 16.0% polyethylene glycol 3350, 2.0% tacsimate pH 8.0, 0.1M tris hydrochloride pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
