3LB6
The structure of IL-13 in complex with IL-13Ralpha2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 73.370, 86.570, 166.788 |
| Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
| Resolution | 82.800 - 3.050 |
| R-factor | 0.2219 |
| Rwork | 0.219 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IL-13 and IL-13Ralpha1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.426 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.800 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.124 | 0.581 |
| Number of reflections | 19978 | |
| <I/σ(I)> | 7.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 100 mM MES, pH 6.0, 200 mM CaCl2, 20% PEG-6000, and 4% v/v polypropylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
