3LAU
Crystal Structure of Aurora2 kinase in complex with a GSK3beta inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04 |
| Detector | ccd |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.070, 45.617, 59.561 |
| Unit cell angles | 90.00, 97.65, 90.00 |
Refinement procedure
| Resolution | 27.700 - 2.100 |
| Rwork | 0.203 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mq4 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 13872 |
| Completeness [%] | 92.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG4000 26% - Tris 100mM pH 8.5 - DTT 5mM - MgCl2 1mM - NaAcetate 200mM, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
