3LAQ
Structure-based engineering of species selectivity in the uPA-uPAR interaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2006-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.421, 137.248, 65.785 |
| Unit cell angles | 90.00, 106.17, 90.00 |
Refinement procedure
| Resolution | 44.740 - 3.200 |
| R-factor | 0.237 |
| Rwork | 0.231 |
| R-free | 0.34100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 17190 | |
| Completeness [%] | 89.5 | 54.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 5-7 % (w/v) PEG 3350, 50 mM Bis-Tris at pH 5.5., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
