3L85
Crystal structure of human NUDT5 complexed with 8-oxo-dGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-09 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.655, 40.008, 98.470 |
| Unit cell angles | 90.00, 121.53, 90.00 |
Refinement procedure
| Resolution | 45.523 - 2.301 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.26100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2dsc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.621 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.426 |
| Number of reflections | 15339 | |
| <I/σ(I)> | 15.8 | 1.75 |
| Completeness [%] | 90.8 | 64.9 |
| Redundancy | 2.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Sodium Acetate, 0.8M NaH2PO4/1.2M K2HPO4 , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
