3L5V

Crystal structure of macrophage migration inhibitory factor (MIF) with glycerol at 1.70A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	67.243, 67.391, 88.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.530 - 1.700
R-factor	0.197
Rwork	0.196
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gcz
RMSD bond length	0.004
RMSD bond angle	0.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	28.530
High resolution limit [Å]	1.700
Number of reflections	43332

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K