3L4Q
Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.950, 98.670, 149.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.970 - 2.300 |
| R-factor | 0.23499 |
| Rwork | 0.232 |
| R-free | 0.29232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GX9 (chain A B) and 2V1Y (chain C D) |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.836 |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.320 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 36826 | |
| Completeness [%] | 99.3 | 98.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
