3L46
Crystal structure of the second BRCT domain of epithelial cell transforming 2 (ECT2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.158, 60.630, 74.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.482 |
R-factor | 0.182 |
Rwork | 0.181 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cou |
RMSD bond length | 0.016 |
RMSD bond angle | 1.551 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.510 |
High resolution limit [Å] | 1.480 | 4.010 | 1.480 |
Rmerge | 0.088 | 0.032 | 0.928 |
Number of reflections | 32244 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 8.5 | 8.1 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.5M sodium citrate, 0.1M TRIS, 1:100 (w/w) subtilisin, pH 7.5, vapor diffusion, hanging drop, temperature 291K |