3L25
Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.947, 84.571, 89.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.000 |
| R-factor | 0.17129 |
| Rwork | 0.169 |
| R-free | 0.20744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fke |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | 0.758 |
| Number of reflections | 41135 | |
| <I/σ(I)> | 15.9 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 3M SODIUM FORMATE, 0.1M SODIUM CITRATE, 5% GLYCEROL, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM FORMATE | ||
| 2 | 1 | 1 | SODIUM CITRATE | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 2 | SODIUM FORMATE | ||
| 5 | 1 | 2 | SODIUM CITRATE | ||
| 6 | 1 | 2 | GLYCEROL |
