3L1Z
Crystal structure of the U-BOX domain of human E4B ubiquitin ligase in complex with UBCH5C E2 ubiquitin conjugating enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 142.708, 142.708, 83.133 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.780 - 3.170 |
| R-factor | 0.2364 |
| Rwork | 0.232 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1L1X AND 3L1Y |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.780 | 3.230 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.192 | 0.780 |
| Number of reflections | 8196 | |
| <I/σ(I)> | 18.06 | 6.18 |
| Completeness [%] | 92.2 | 96 |
| Redundancy | 58.8 | 57.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 4M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
