3L1P
POU protein:DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 |
| Unit cell lengths | 49.250, 49.250, 265.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.430 - 2.800 |
| R-factor | 0.22618 |
| Rwork | 0.224 |
| R-free | 0.25713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PBD ENTRY 1HF0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.430 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.079 | 0.671 |
| Number of reflections | 15237 | |
| <I/σ(I)> | 10.08 | 1.89 |
| Completeness [%] | 98.4 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
