3L0O

Structure of RNA-free Rho transcription termination factor from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-17
Detector	MAR CCD 165 mm
Wavelength(s)	0.931
Spacegroup name	P 43 2 2
Unit cell lengths	139.000, 139.000, 150.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 2.350
R-factor	0.26934
Rwork	0.268
R-free	0.29716
Structure solution method	SIRAS
RMSD bond length	0.013
RMSD bond angle	2.004
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	81012
Completeness [%]	99.2	90.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	295	0.1M sodium acetate, 0.8M magnesium sulphate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	4.4	295	0.1M sodium acetate, 0.8M magnesium sulphate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K