3KZ8
Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs (p53-DNA complex 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97620 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.422, 49.812, 68.056 |
| Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
| Resolution | 34.540 - 1.910 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chain A of 2AC0 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.540 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 37958 | |
| <I/σ(I)> | 25.3 | 4.67 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.20M AMMONIUM IODIDE, 20% PEG 3350, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
