3KZ0

MCL-1 complex with MCL-1-specific selected peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 2
Unit cell lengths	64.325, 121.523, 46.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.171 - 2.349
R-factor	0.224
Rwork	0.222
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pqk
RMSD bond length	0.004
RMSD bond angle	0.662
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.4_129)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.170
High resolution limit [Å]	2.350
Number of reflections	15790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	0.2 M zinc sulfate, 0.1 M imidazole, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K