3KX2

Crystal structure of Prp43p in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-08-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.980
Spacegroup name	P 32 2 1
Unit cell lengths	118.152, 118.152, 253.601
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.423 - 2.200
R-factor	0.2085
Rwork	0.206
R-free	0.24970
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.012	0.750
Number of reflections	104418
Completeness [%]	99.5	99.6
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.75	277	100mM Mes-NaOH, pH 6.75, 14% PEG 8000, 300mM NaOAc, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.75	277	100mM Mes-NaOH, pH 6.75, 14% PEG 8000, 300mM NaOAc, VAPOR DIFFUSION, HANGING DROP, temperature 277K