3KUT
Crystal structure of the MLLE domain of poly(A)-binding protein in complex with the binding region of Paip2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.309, 31.748, 48.454 |
| Unit cell angles | 100.29, 90.37, 99.05 |
Refinement procedure
| Resolution | 47.620 - 1.500 |
| R-factor | 0.16917 |
| Rwork | 0.168 |
| R-free | 0.20065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i2t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.125 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.233 |
| Number of reflections | 22328 | |
| <I/σ(I)> | 20.8 | 3.4 |
| Completeness [%] | 96.6 | 93.2 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 2.25M ammonium sulfate, 0.2M KBr, 0.1 M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
