3KTU
Structure of human 8-oxoGuanine Glycosylase 1 bound to fluorninated oxoG-containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.400, 92.400, 212.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.210 - 2.300 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ebm |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.112 | 0.397 |
Number of reflections | 24522 | |
<I/σ(I)> | 14.3 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 9.2 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 277 | Calcium Chloride, Sodium Cacodylate, pH 6.0, PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Calcium Chloride | ||
2 | 1 | 2 | Calcium Chloride | ||
3 | 1 | 1 | Sodium Cacodylate | ||
4 | 1 | 2 | Sodium Cacodylate | ||
5 | 1 | 1 | PEG 8000 | ||
6 | 1 | 2 | PEG 8000 |