3KTP
Structural basis of GW182 recognition by poly(A)-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 38.461, 38.461, 136.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.010 - 1.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.23067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i2t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.294 |
| Number of reflections | 16216 | |
| <I/σ(I)> | 34.1 | 3.4 |
| Completeness [%] | 98.5 | 82.7 |
| Redundancy | 9.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 2.0 M ammonium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
